|
|||||
|
|
Phosphorylation of filamin and other proteins in cultured fibroblasts. |
|
| Authors: | P Davies Y Shizuta K Olden M Gallo I Pastan |
| Affiliation: | National Institutes of Health National Cancer Institute Laboratory of Molecular Biology Bethesda, Maryland 20014 USA |
| Abstract: | Incubation of subcellular fractions of fibroblasts with [32P]ATP demonstrated 10 phosphoproteins whose phosphorylation can be increased by cyclic AMP or cyclic AMP-dependent protein kinase. One of these phosphoproteins, MW 240,000, resembles the actin binding protein, filamin, and can be selectively precipitated by antibodies to chicken gizzard filamin. Furthermore chicken gizzard filamin can be phosphorylated by skeletal muscle protein kinase and cyclic AMP stimulates this reaction. |
| Keywords: | cyclic AMP adenosine 3′:5′-cyclic monophosphate EGTA ethylene glycol bis(β-aminoethylether)-N,N′-tetraacetic acid MES 2-(N-morpholino)ethanesulfonic acid SDS sodium dodecyl sulfate NRK normal rat kidney fibroblasts |
| 本文献已被 ScienceDirect 等数据库收录！ | |