Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein. |
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Authors: | W Witke W Nellen and A Noegel |
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Institution: | Max-Planck-Institut für Biochemie, Martinsried, FRG. |
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Abstract: | Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3' portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene. |
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