Transformation of eggplant (Solanum melongena L.) using a binary Agrobacterium tumefaciens vector |
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Authors: | G L Rotino S Gleddie |
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Institution: | (1) Plant Research Centre, Agriculture Canada, K1A OC6 Ottawa, Ontario, Canada;(2) Present address: Istituto Sperimentale per l'Orticoltura, CP 48, I-84098 Pontecagnano (SA), Italy |
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Abstract: | Summary Kanamycin resistant plants of Solarium melongena L. (eggplant) cv. Picentia were obtained following the cocultivation of leaf explants with Agrobacterium tumefaciens. A disarmed binary vector system containing the neomycin phosphotransferase (NPTII) gene as the selectable marker and chloramphenicol acetyltransferase (CAT) as a reporter gene was utilized. In vitro grown plants were used as sources of explants to produce transgenic plants on selective medium containing 100 mg/l kanamycin. The transformation and expression of the foreign genes was confirmed by DNA hybridizations, leaf disc assays, and by measuring NPTII and CAT enzyme activities. This technique is simple, rapid, efficient, and transgenic eggplants of this commercial cultivar have been transferred to soil where they have flowered and set seed.Abbreviations CAT
chloramphenicol acetyltransferase
- MS
Murashige and Skoog
- NPTII
neomycin phosphotransferase
- NOS
nopaline synthase
- ZEA
zeatin |
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Keywords: | Solanum melongena eggplant Agrobacterium tumefaciens kanamycin transformation shoot regeneration |
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