Influence of IAA,TDZ, and light quality on asymbiotic germination,protocorm formation,and plantlet development of <Emphasis Type="Italic">Cyrtopodium glutiniferum</Emphasis> Raddi., a medicinal orchid |
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Authors: | Ingrid N Vogel Andrea F Macedo |
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Institution: | 1.Laboratório de Cultura de Células, Tecidos Vegetais e Investiga??o Fitoquímica, Curso de Farmácia,Universidade Estácio de Sá,Rio de Janeiro,Brazil;2.Laboratório Integrado de Biologia Vegetal, Departamento de Botanica, Instituto de Biociências,CCBS, Universidade Federal do Estado do Rio de Janeiro,Rio de Janeiro,Brazil;3.Urca, Rio de Janeiro,Brazil |
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Abstract: | We investigated the influence of light quality on in vitro germination and protocorm formation, and the effect of indole-3-acetic
acid (IAA) and thidiazuron (TDZ) on proliferation of protocorm-like bodies (PLBs) and development of plantlets of Cyrtopodium glutiniferum Raddi. Germination was faster under white and blue light, and highest under green light. The protocorm developed more rapidly
under white, blue, and green light. Continuous darkness delayed seed germination and reduced protocorm formation. Among the
plant growth regulators (PGRs) tested for multiplying PLBs, shoots, and roots from protocorms, IAA proved to be superior.
TDZ was effective in inducing PLB fresh weight accumulation, but not morphogenesis, unlike IAA. This study indicated that
C. glutiniferum seedlings can be produced in vitro using asymbiotic seed germination techniques. High germination rate and protocorm yield
can be obtained by initially cultivating C. glutiniferum seeds on medium without growth regulators under white light, or under white light supplemented with green or blue light.
This culture system complies with commercial and conservation requirements for rapid and low-cost propagation. |
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