Use of rubidium, manganese, and zinc as tracers to measure intestinal permeability by PIXE analysis |
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Authors: | Keitaro Nakao Yasuo Suzuki Hitoshi Imaseki Hisamasa Joshima Itsuro Tamanoi Yasushi Saito |
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Institution: | (1) Department of Clinical Cell Biology (F5), Graduate School of Medicine, Chiba University, Chiba, Japan;(2) National Institute of Radiological Sciences, Chiba, Japan |
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Abstract: | Intestinal permeability has been suggested to be closely linked with the etiology or activity of Crohn’s disease. However,
current methods for measurement of intestinal permeability are too laborious for routine examination, as they require urine
collection and/or use of radioisotopes. The present study was performed to develop a more convenient and safer method for
assessing intestinal permeability using blood samples rather than urine. Rats with indomethacin-induced enteritis were orally
administered Rb, Mn, and Zn as tracers. Intestinal permeability was determined by assaying the levels of Rb, Mn, and Zn in
blood samples by particle-induced X-ray emission (PIXE). The distributions of Rb, Mn, and Zn in the small intestine after
administration were analyzed by micro-PIXE. The conventional PIXE analysis showed that the levels of Rb and Zn in the blood
in the enteritis group were correlated with the grade of enteritis. The micro-PIXE analysis showed that Rb, Mn, and Zn were
translocated into the wall of the proximal small intestine 5 min after administration, and this effect was more conspicuous
in the enteritis group than in controls. Analysis of blood or small intestine tissue samples using the PIXE allows determination
of both intestinal permeability and the route of permeation. |
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Keywords: | Crohn’ s disease indomethacin intestinal permeability PIXE analysis |
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