Effect of inducers on the production of 5-aminolevulinic acid by recombinant Escherichia coli |
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Authors: | Xiaoxia Liu Jianping Lin Peilin Cen |
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Affiliation: | Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou, P. R. China. |
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Abstract: | Aminolevulinic acid (ALA) was produced by recombinant Escherichia coli BL21(DE3) (pET28-A.R-hemA) harboring the ALA synthase gene (hemA) from Agrobacterium radiobacter zju-0121. The effects of inducers on the ALA synthase activity and ALA productivity were evaluated. The results indicated that a low isopropyl-beta-D-thiogalactoside (IPTG) concentration (0.05 mmol/L) was favorable for high expression of ALA synthase, which resulted in higher ALA productivity. For metabolic engineering applications, lactose was a better substitute of IPTG for active enzyme expression. When lactose concentration was 5 mmol/L, the specific ALA synthase activity and ALA productivity reached 16.7 nmol/(min . mg of protein) and 1.15 g/L, respectively, which were about 15% and 43% higher than those induced by IPTG. |
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