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Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry |
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| Authors: | Ishimura Yuzuru Gao Ying Tong Panda Satya P Roman Linda J Masters Bettie Sue S Weintraub Susan T |
| Institution: | Department of Biochemistry, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA. |
| Abstract: | Using headspace gas chromatography-mass spectrometry, we detected significant amounts of nitrous oxide in the reaction products of the monooxygenase reaction catalyzed by neuronal nitric oxide synthase. Nitrous oxide is a dimerization product of nitroxyl anion; its presence in the reaction products indicates that the nitroxyl anion is a product of the neuronal nitric oxide synthase-catalyzed reaction. |
| Keywords: | Gas chromatography-mass spectrometry Nitric oxide synthase Nitric oxide Nitroxyl |
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