Production and characterization of KDO-specific monoclonal antibodies recognizing lipopolysaccharides from heptoseless mutants of Salmonella |
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Authors: | Siv M. Lind Nils I.A. Carlin Alf A. Lindberg |
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Affiliation: | Department of Antibacterial Chemotherapy, Research and Development Laboratories, Astra Läkemedel AB, S-151 85 Södertälje, Sweden;Department of Bacteriology, National Bacteriological Laboratory, S-105 21 Stockholm, Sweden;Karolinska Institute, Department of Clinical Bacteriology, Huddinge University Hospital, S-141 86 Huddinge, Sweden |
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Abstract: | Abstract Hybrid cell lines producing monoclonal antibodies with specificity for the lipopolysaccharide (LPS) from the deep rough mutant Salmonella minnesota R595 have been established. Spleen cells from BALB/c mice immunized with live R595 bacteria were fused with Sp 2/0 myeloma cells and three hybridomas producing antibodies specific for heptoseless LPS from Salmonella were selected. All three monoclonal antibodies were shown to bind only to heptoseless, but 3-deoxy- d -manno-octulosonic acid (KDO) containing LPS when tested in enzyme-linked immunosorbent assay (ELISA) against a set of structurally defined LPS and lipid A from Salmonella, Shigella and Escherichia coli . Synthetic KDO was an efficient inhibitor of the antibody-R595 LPS interaction defining that KDO is in an immunodeterminant position interacting with the monoclonal antibodies. |
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