首页 | 本学科首页   官方微博 | 高级检索  
     

沙眼衣原体感染诱导小鼠生殖道局部促炎性细胞因子的表达情况
引用本文:陈凡,张宁洁,余平,程文. 沙眼衣原体感染诱导小鼠生殖道局部促炎性细胞因子的表达情况[J]. 微生物学杂志, 2013, 0(6): 70-74
作者姓名:陈凡  张宁洁  余平  程文
作者单位:[1] 中南大学湘雅医院 心血管内科,湖南 长沙,410078 [2] 中南大学基础医学院 免疫学系,湖南 长沙,410008
基金项目:国家自然科学基金(81071403);湖南省自然科学基金(10JJ3093)
摘    要:建立小鼠生殖道沙眼衣原体感染模型,观察小鼠生殖道局部促炎性细胞因子的表达。将小鼠生物型沙眼衣原体C. muridarum 1×104 IFU阴道接种于C57B6背景雌性小鼠,取感染后阴道拭子做沙眼衣原体培养,计算IFU,监测小鼠感染和病原体清除情况;80 d后处死小鼠,检测子宫输卵管病理改变;ELISA检测感染过程中小鼠生殖道促炎性细胞因子IL-1α、IL-6、MIP-2和TNF-α产生情况。小鼠感染在第3至第15天维持较高水平,然后病原体被逐渐清除,整个病程约3~5周;病理检测显示子宫输卵有严重炎症、管腔扩张积水,狭窄等;于感染后第3天检测到局部IL-1α、IL-6、MIP-2分泌,第7天达高峰,然后逐渐下降至正常水平( IL-6于11 d恢复正常,IL-1α和 MIP-2于15 d恢复正常)。 TNF-α仅在第7天检测到高水平表达。相对于TNF-α和IL-6,IL-1α和MIP-2维持时间较长。成功建立沙眼衣原体感染小鼠生殖道模型,沙眼衣原体急性感染可诱导小鼠生殖道局部分泌IL-1α、IL-6、MIP-2和TNF-α。

关 键 词:沙眼衣原体  生殖道感染  促炎性细胞因子

Expression of Inflammation Promotive Cytokines of Local Induction Infected by Chlamydia trachomatis biotype mouse in Mice Genital Tract
CHEN Fan,ZHANG Ning-jie,YU Ping and CHENG Wen. Expression of Inflammation Promotive Cytokines of Local Induction Infected by Chlamydia trachomatis biotype mouse in Mice Genital Tract[J]. Journal of Microbiology, 2013, 0(6): 70-74
Authors:CHEN Fan  ZHANG Ning-jie  YU Ping  CHENG Wen
Affiliation:CHEN Fan, ZHANG Ning-jie, YU Ping, CHENG Wen
Abstract:Model of mouse genital tract infected by C. trachomatis was established to observe the expression of in-flammatory promotive cytokines in mice genital tract. Female mice of C57B6 background were inoculation with C. tra-chomatis biotype mouse intravaginally at 1í104 IFU. The swabs were then collected from mice vaginal tracts and cul-tured for C. trachomatis and calculated the IFU to monitor the situation of the infection and the removal of the patho-gen in mice. 80 days after the infection the mice were killed and tested for pathological changes in their wombs and oviducts. Tested for their production of inflammatory promotive cytokines IL-1α, IL-6, MIP-2, and TNF-α with ELISA determination during the infection process. The results showed that at day 3 through day 15 the infection was maintained at fairly high level, then the pathogen was gradually removed, the course of the disease was about 3~5 weeks;the pathogenic tests showed that the oviduct had serious inflammation, and the uterus cavity was seriously di-lated and had serious hydrops and stenosis etc. The early local secretion of IL-1α, IL-6 and MIP-2 could be detected on day 3 after the infection, then rise to the highest level at day 7, and gradually decreased to normal level on day 11 (IL-6) and day 15 (IL-1αand MIP-2). TNF-αcould be detected only on day 7 with a high level. As compared with TNF-αand IL-6, IL-1αand MIP-2 maintained for a longer time. The establishment of model of C. trachomatis to in-fect mouse genital tract was successful, C. trachomatis acute infection could induce mouse genital tract to excrete lo-cally IL-1α, IL-6, MIP-2 and TNF-α.
Keywords:Chlamydia trachomatis  genital infection  inflammatory promotive cytokine
本文献已被 维普 等数据库收录!
点击此处可从《微生物学杂志》浏览原始摘要信息
点击此处可从《微生物学杂志》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号