Calculation and measurement of semiconduction activation energy and electron mobility in cytochrome oxidase,with evidence that charge carriers are polarons,which may couple oxidation to phosphorylation |
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Authors: | Freeman W. Cope Karl David Straub |
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Affiliation: | (1) Biochemistry Division, Aerospace Medical Research Laboratory, U.S. Naval Air Development Center, 18974 Warminster, Pennsylvania;(2) Biochemistry Department, Duke University, Durham, North, Carolina |
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Abstract: | It is shown that an electron transport reaction which is rate-limited by electron conduction 4 across a solid biological particle or membrane in accord with Ohm's law should have a first order rate constant approximately proportional to exp (E a/KT ), whereT is absolute temperature,k is the Boltzmann constant andE a is the activation energy for semiconduction in the solid particle, where resistance in the semiconductor is proportional to exp (E a /KT). For two different preparations of cytochrome oxidase, this method yields an average value ofE a =0.27 ev, which agrees well with direct conductivity measurements on dry solid enzyme, which provide an average value ofE a =0.26 ev. Electron mobility in dry cytochrome oxidase is estimated to be approximately gm=10t-5 cm2 voltt-1 sect-1. Elovich decay of current in dry cytochrome oxidase was observed, which parallels the Elovich kinetics of cytochrome oxidase activity in yeast observed previously by M:uhlig (1966). Finally, the solid state kinetic theory is used to deduce that conduction of polarons may be involved in cytochrome oxidase activity (1 polaron=1 electron + 1 phonon), which provides a link with the solid state phonon phosphorylation theory of Straub. |
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