A simple and rapid method for analyzing the Monascus pigment-mediated degradation of mutagenic 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole by in-capillary micellar electrokinetic chromatography. |
| |
Authors: | T Watanabe T K Mazumder A Yamamoto S Nagai S Arimoto-Kobayashi H Hayatsu S Terabe |
| |
Affiliation: | Yaegaki Technology Development Laboratories, Yaegaki Bio-industry, 681 Hayashida, Himeji, Japan. |
| |
Abstract: | A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4, 3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to their different migration velocities, mix for a certain period of time to interact, and then they are separated and quantified. Using this technique, we were able to demonstrate that Trp-P-2(NHOH) is degraded by the pigment. The degradation was pigment-dose dependent, and because the pigment was recovered unchanged, it was deduced that the pigment acted catalytically for the degradation. The entire MEKC procedure takes 8 min. |
| |
Keywords: | |
|
|