Estimation of the diversity between DNA calorimetric profiles,differential melting curves and corresponding melting temperatures |
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| Authors: | Chun‐Ling Chang Alexander S Fridman Inessa E Grigoryan Elena N Galyuk Oleg N Murashko Chin‐Kun Hu Dmitri Y Lando |
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| Institution: | 1. Institute of Physics, Academia Sinica, Nankang, Taipei, Taiwan;2. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus;3. Department of Physics, Yerevan State University, Yerevan, Armenia;4. Department of Bioorganic Chemistry, Belarusian State Medical University, Minsk, Belarus;5. Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan;6. National Center for Theoretical Sciences, National Tsing Hua University, Hsinchu, Taiwan;7. Business School, University of Shanghai for Science and Technology, Shanghai 200093, China |
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| Abstract: | The Poland–Fixman–Freire formalism was adapted for modeling of calorimetric DNA melting profiles, and applied to plasmid pBR 322 and long random sequences. We studied the influence of the difference (HGC?HAT) between the helix‐coil transition enthalpies of AT and GC base pairs on the calorimetric melting profile and on normalized calorimetric melting profile. A strong alteration of DNA calorimetrical profile with HGC?HAT was demonstrated. In contrast, there is a relatively slight change in the normalized profiles and in corresponding ordinary (optical) normalized differential melting curves (DMCs). For fixed HGC?HAT, the average relative deviation (S) between DMC and normalized calorimetric profile, and the difference between their melting temperatures (Tcal?Tm) are weakly dependent on peculiarities of the multipeak fine structure of DMCs. At the same time, both the deviation S and difference (Tcal?Tm) enlarge with the temperature melting range of the helix‐coil transition. It is shown that the local deviation between DMC and normalized calorimetric profile increases in regions of narrow peaks distant from the melting temperature. |
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| Keywords: | Differential scanning calorimetry DNA differential melting curve DNA helix‐coil transition high‐resolution calorimetric melting profiles complex DNA systems |
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