Determination of the sensitivity of bacteria to barium ions,a taxonomic marker of the genus <Emphasis Type="Italic">Pseudomonas</Emphasis> |
| |
Authors: | E P Sivolodskii |
| |
Institution: | 1.Kirov Military Medical Academy,St. Petersburg,Russia |
| |
Abstract: | Comparative efficacy of the determination of the sensitivity of bacterial cells to barium ions was evaluated on a synthetic
nutrient medium, FMH agar, Mueller-Hinton agar, and AGV agar. The synthetic nutrient medium developed for this study contained
L-proline and L-glutamine as the sole nitrogen and carbon source, which promoted growth of all Pseudomonas strains and ensured the minimal level of barium binding. The sensitivity of 80 strains belonging to 11 Pseudomonas species, including the type strains, as well as of 80 strains of 22 other bacterial species, was studied. The sensitivity
of bacteria to barium ions was determined by using serial dilutions of barium chloride in the nutrient medium. The highest
level of analytical sensitivity of pseudomonads to barium ions was determined on the synthetic nutrient medium: the minimal
inhibitory concentration (MIC) values of barium chloride ranged from 0.5 to 6 g/L, the MIC90 value was 2 g/L. At the same time, 86.1% of all strains of fluorescent Pseudomonas species produced fluorescein on the control BaCl2-free synthetic nutrient medium. For representatives of other genera grown on all the studied nutrient media, the MIC values
of barium chloride ranged from 20 to 50 g/L. The proposed method for determination of the sensitivity of bacteria to barium
ions using the synthetic nutrient medium with 6 g/L of barium chloride as a criterion for the classification of barium-sensitive
strains to the genus Pseudomonas is suitable for standardization. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|