Application of low temperatures during photoinhibition allows characterization of individual steps in photodamage and the repair of photosystem II |
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Authors: | Prasanna Mohanty Suleyman I Allakhverdiev Norio Murata |
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Institution: | (1) Jawaharlal Nehru University, New Delhi, India;(2) Regional Plant Resource Center, Nayapalli, Bhubaneswar, 751015, India;(3) Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia;(4) National Institute for Basic Biology, Okazaki 444-8585, Japan |
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Abstract: | Recent investigations of photoinhibition have revealed that photodamage to photosystem II (PSII) involves two temporally separated
steps: the first is the inactivation of the oxygen-evolving complex by light that has been absorbed by the manganese cluster
and the second is the impairment of the photochemical reaction center by light that has been absorbed by chlorophyll. Our
studies of photoinhibition in Synechocystis sp. PCC 6803 at various temperatures demonstrated that the first step in photodamage is not completed at low temperatures,
such as 10°C. Further investigations suggested that an intermediate state, which is stabilized at low temperatures, might
exist at the first stage of photodamage. The repair of PSII involves many steps, including degradation and removal of the
D1 protein, synthesis de novo of the precursor to the D1 protein, assembly of the PSII complex, and processing of the precursor
to the D1 protein. Detailed analysis of photodamage and repair at various temperatures has demonstrated that, among these
steps, only the synthesis of the precursor to D1 appears to proceed at low temperatures. Investigations of photoinhibition
at low temperatures have also indicated that prolonged exposure of cyanobacterial cells or plant leaves to strong light diminishes
their ability to repair PSII. Such non-repairable photoinhibition is caused by inhibition of the processing of the precursor
to the D1 protein after prolonged illumination with strong light at low temperatures. |
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Keywords: | D1 protein Low temperature Photodamage Photoinhibition Photosynthesis Photosystem II Processing Repair |
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