| 钙离子对鼠角质细胞生长和分化的影响 |
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| 引用本文： | 于海强,周燕,华平,谭文松.钙离子对鼠角质细胞生长和分化的影响[J].生物工程学报,2002,18(5):626-629. |
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| 作者姓名： | 于海强 周燕 华平 谭文松 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 基金项目： | 国家高技术研究发展计划课题 (No .2 0 0 1AA2 10 6 41)，国家重点基础研究发展规划“组织工程的基本科学问题”项目(No .G19990 5 430 9)的资助~~ |
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| 摘 要： | 用无血清培养基培养角质细胞，研究了Ca2+对鼠角质细胞生长和分化的影响。实验结果表明，培养基中钙离子最佳浓度为0.2mmol/L。在此浓度下，细胞克隆形成率达到10.8%，细胞的贴壁率达到28.7%，细胞的分化比例和老化比例分别为5.4%和26.3%；当Ca2+浓度达到0.6mmol/L以上时，则会引起角质细胞显著的分化和老化。
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| 关 键 词： | 角质细胞， 细胞培养， 细胞分化， 细胞老化 |
| 文章编号： | 1000-3061(2002)05-0626-04 |
| 修稿时间： | 2002年3月22日 |
Calcium-regulated Growth and Differentiation of the Mouse Epidermal Keratinocytes |
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| YU Hai-Qiang,ZHOU Yan,HUA Ping,TAN Wen-Song.Calcium-regulated Growth and Differentiation of the Mouse Epidermal Keratinocytes[J].Chinese Journal of Biotechnology,2002,18(5):626-629. |
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| Authors: | YU Hai-Qiang ZHOU Yan HUA Ping TAN Wen-Song |
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| Institution: | State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 200237, China. |
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| Abstract: | The effect of the calcium ion (Ca 2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8 %, 30.8 %, 5.1 %, and 26.8 %, respectively. However, the Ca 2+ concentrations in the medium above 0.6mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro. |
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| Keywords: | keratinocytes cell culture cell differentiation cell senescence |
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