Cryoprotection of some rat heart enzymes |
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| Authors: | R Hart L J Ramazzotto R Engstrom |
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| Institution: | Department of Physiology, Fairleigh Dickinson University, Hackensack, New Jersey 07601 U.S.A. |
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| Abstract: | Rat heart muscle was spectrophotometrically assayed for succinic dehydrogenase and cytochrome oxidase activity to determine the extent of cryoprotection afforded by DMSO-glycerol solutions. Activities of these two enzymes were greater in the frozen untreated heart than in the normal heart. It was also found that the cryoprotectant without freezing tended to increase the activity of succinic dehydrogenase while cytochrome oxidase activity decreased. |
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