Visualization and quantification of <Emphasis Type="Italic">Plasmodium falciparum</Emphasis> intraerythrocytic merozoites |
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Authors: | Swati Garg Shalini Agarwal Surbhi Dabral Naveen Kumar Seema Sehrawat Shailja Singh |
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Institution: | 1.Malaria Group,International Centre for Genetic Engineering and Biotechnology (ICGEB),New Delhi,India;2.Department of Life Sciences,Shiv Nadar University,Gautam Budha Nagar,India |
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Abstract: | Malaria, a leading parasitic killer, is caused by Plasmodium spp. The pathology of the disease starts when Plasmodium merozoites infect erythrocytes to form rings, that matures through a large trophozoite form and develop into schizonts containing multiple merozoites. The number of intra-erythrocytic merozoites is a key-determining factor for multiplication rate of the parasite. Counting of intraerythrocytic merozoites by classical 2-D microscopy method is error prone due to insufficient representation of merozoite in one optical plane of a schizont. Here, we report an alternative 3-D microscopy based automated method for counting of intraerythrocytic merozoites in entire volume of schizont. This method offers a considerable amount of advantages in terms of both, ease and accuracy. |
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