小鼠早期胚胎发育过程中TGF-α和EGF-R mRNA的表达

This study was conducted to examine the activity of TGF α and EGF R mRNA using the RT PCR technique in the early embryonic development of the mouse. Expression of TGF α mRNA was initially observed in the 8 cell stage embryo of the mouse. The level of expression gradually increased in the morula and blastula. Expression of EGF R mRNA was initially observed in the 4 cell stage embryo of the mouse and 8 cell stage embryo;morula and blastula all expressed EGF R mRNA and the levels of expression had not changed. These results confirm that epidermal growth factors play a key role in early embryonic development of the mouse, especially TGF α which can stimulate and regulate embryonic development via the autocrine system.