Determination of 3-deoxy-D-manno-octulosonic acid (KDO), N-acetylneuraminic acid, and their derivatives by ion-exchange liquid chromatography |
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| Authors: | A K Shukla R Schauer F M Unger U Z?hringer E T Rietschel H Brade |
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| Affiliation: | 1. Department of Medicine II, University Medical Center Mainz, Johannes Gutenberg-University Mainz, Germany;2. Centrum for Thrombosis and Haemostasis, University Medical Center Mainz, Johannes Gutenberg-University Mainz, Germany;3. Department of Internal Medicine, St. Vincenz and Elisabeth Hospital Mainz (KKM), Germany;4. Department of Radiology and Nuclear Medicine, Catholic Clinic Mainz (KKM), Germany;5. Department of Diagnostic and Interventional Radiology, University Clinic, Johann Wolfgang Goethe-University Frankfurt/Main, Germany;1. Department of Internal Medicine, Taichung Hospital, Ministry of Health and Welfare, Taichung, Taiwan;2. Department of Healthcare Administration, Central Taiwan University of Science and Technology, Taichung, Taiwan;3. Management Office for Health Data, China Medical University Hospital, Taichung, Taiwan;4. Graduate Institute of Clinical Medical Science and School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan;5. Department of Nuclear Medicine and PET Center, China Medical University Hospital, Taichung, Taiwan;1. Center for Thrombosis and Hemostasis, University Medical Center of the Johannes Gutenberg University, Mainz, Germany;2. Department of Cardiology, Democritus University of Thrace, Alexandroupolis, Greece;1. Department of Cardiology and Internal Medicine B, University Medicine Greifswald, Germany;2. DZHK (German Centre for Cardiovascular Research), partner site Greifswald, Germany;3. Institute for Community Medicine, Section Epidemiology of Health Care and Community Health, University Medicine Greifswald, Germany;4. Institute for Pathology and Neuropathology, Department of Molecular Pathology, University of Tübingen, Germany;5. Departement of Cardiology, Universitätsklinikum Hamburg Eppendorf (UKE), Germany;6. DZHK (German Centre for Cardiovascular Research), partner site Hamburg/Kiel/Lübeck, Germany;1. Department of Neurology, The Adelaide and Meath Hospital incorporating the National Children''s Hospital, Trinity College, Dublin, Ireland;2. Stroke Service, The Adelaide and Meath Hospital, incorporating the National Children''s Hospital, Trinity College, Dublin, Ireland;3. Stroke Research Group, UCL Institute of Neurology, The National Hospital for Neurology & Neurosurgery, UK;4. Department of Clinical Neurosciences, Royal Free Campus, UCL Institute of Neurology, The National Hospital for Neurology & Neurosurgery, UK;5. School of Immunity and Infection, University of Birmingham Medical School, UK;6. Haemostasis Research Unit, University College, London, UK;7. Dept. of Haematology, University College, London, UK |
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| Abstract: | A liquid chromatography (1.6 MPa) system for the analysis of 3-deoxy-D-manno-2-octulosonic acid (KDO), N-acetylneuraminic acid (Neu5Ac), methyl alpha- and beta-glycosides of Neu5Ac and KDO, alpha-heptosyl-(1----5)-KDO, various sialyllactoses, alpha-KDO-(2----4)-KDO, alpha-KDO-(2----4)-KDO methyl alpha-glycoside, beta-KDO-(2----4)-KDO methyl beta-glycoside, D-glucuronic acid, D-glucurono-3,6-lactone, and D-galacturonic acid has been developed. Separation was achieved within 10 and 30 min by the use of a small column filled with a strongly basic, anion-exchange resin, Aminex A-29, and 0.75 or 10mM sodium sulfate solutions as mobile phases. This method allowed the determination of KDO and sialic acids in amounts of 100 ng (0.5 nmol) and 200 pg (0.6 pmol), respectively. |
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