| 柳州和南宁HIV-1亚型分布及耐药情况调查 |
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| 引用本文： | 苏齐鉴,周平,闭志友,肖信,吴书志,岑平,邓伟,梁浩.柳州和南宁HIV-1亚型分布及耐药情况调查[J].病毒学报,2010,26(4). |
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| 作者姓名： | 苏齐鉴 周平 闭志友 肖信 吴书志 岑平 邓伟 梁浩 |
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| 作者单位： | 广西医科大学,公共卫生学院&艾滋病研究中心,南宁,530021;广西中医学院,附属瑞康医院,广西中医(中西医结合)艾滋病研究中心,南宁,530011;柳州市疾病预防控制中心,结核病防治所,柳州,545001;广西医科大学,公共卫生学院&艾滋病研究中心,南宁,530021 |
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| 基金项目： | 国家自然科学基金项目(30760218); 广西卫生厅重点科研课题(200628) |
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| 摘 要： | 为了解柳州和南宁两市HIV-1亚型分布和耐药情况,在柳州和南宁招募HIV感染者和AIDS患者共304名,采集外周静脉血,从血浆中提取HIVRNA,扩增HIVpol基因并测序。将获得的序列进行系统进化树分析,结果表明柳州的HIV-1毒株中存在CRF01_AE和CRF07_BC两种亚型,其中CRF01_AE毒株占75.2%,CRF07_BC毒株占24.8%;南宁的HIV-1毒株中存在CRF01_AE、CRF08_BC、B亚型和C亚型共4种亚型,其中CRF01_AE和CRF08_BC仍是南宁最主要的亚型,CRF01_AE占85.8%,CRF08_BC占11.5%。根据所得的序列资料进行HIV-1耐药性分析,计算耐药率。计算结果表明,柳州未治疗和治疗研究对象的耐药率分别为3.3%和8.7%,南宁未治疗和治疗研究对象的耐药率分别为1.4%和27.5%。
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| 关 键 词： | 人类免疫缺陷病毒1型 亚型 耐药性 |
Distribution of HIV-1 Subtype and Prevalence of HIV-1 Drug Resistance in Liuzhou and Nanning |
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| SU Qi-jian,ZHOU Ping,BI Zhi-you,XIAO Xin,WU Shu-zhi,CEN Ping,DENG Wei,LIANG Hao.Distribution of HIV-1 Subtype and Prevalence of HIV-1 Drug Resistance in Liuzhou and Nanning[J].Chinese Journal of Virology,2010,26(4). |
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| Authors: | SU Qi-jian ZHOU Ping BI Zhi-you XIAO Xin WU Shu-zhi CEN Ping DENG Wei LIANG Hao |
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| Institution: | SU Qi-jian1,2,ZHOU Ping3,BI Zhi-you1,XIAO Xin1,WU Shu-zhi1,CEN Ping1,DENG Wei1,LIANG Hao1(1.Center for AIDS Research & School of Public Health,Guangxi Medical University,Nanning 530021,China,2.Center for AIDS Research,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medicine University,Nanning 530011,3.Institution of Tuberculosis Control and Prevention,Liuzhou Center for Disease Control and Prevention,Liuzhou 545001,China) |
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| Abstract: | To investigate HIV-1 subtype distribution and prevalence of HIV-1 drug resistance in Liuzhou and Nanning,a total of 304 HIV-infected subjects or AIDS patients from Liuzhou and Nanning were recruited.Whole blood was withdrawn from a peripheral vein of each subject.HIV RNA were extracted from plasma,and subjected to PCR amplification targeting HIV pol gene fragment and DNA sequencing.Sequences obtained were subtyped by phylogenetic analysis.Two subtypes,CRF01_AE and CRF07_BC,were found in subjects from Liuzho... |
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| Keywords: | human immunodeficiency virus type 1 subtype drug resistance |
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