| 中国小反刍兽疫病毒分离株China/Tib/07全基因组序列测定与分析 |
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| 引用本文： | 刘文华,包静月,吴晓东,王志亮.中国小反刍兽疫病毒分离株China/Tib/07全基因组序列测定与分析[J].病毒学报,2010(4). |
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| 作者姓名： | 刘文华 包静月 吴晓东 王志亮 |
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| 作者单位： | 扬州大学兽医学院;中国动物卫生与流行病学中心国家外来动物疫病诊断中心;青岛农业大学动物科技学院; |
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| 基金项目： | 农业部948项目(2006-G57(4)B) |
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| 摘 要： | 小反刍兽疫病毒属于副黏病毒科麻疹病毒属成员。本研究对我国首次分离的小反刍兽疫病毒株China/Ti-bet/07进行了全基因组序列测定及分子生物学特征分析。根据GenBank公布的小反刍兽疫病毒基因组序列设计引物通过RT-PCR扩增病毒基因组内部序列,通过3′和5′-RACE获得病毒基因组末端序列。序列测定与分析的结果表明,China/Tib/07株全长15948bp,预测编码6种结构蛋白和2种非结构蛋白,与已发表小反刍兽疫病毒基因组的长度和结构相似;在系统进化上与西南亚流行毒株有很高的同源性(91.6%～98.1%);与麻疹病毒属的其它成员相比,与牛瘟病毒的同源性最高(64.3%)。
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| 关 键 词： | 小反刍兽疫病毒 麻疹病毒属 编码区 全基因组 核酸序列 |
Genome Sequencing and Analysis of a Peste Des Petits Ruminants Virus Isolate,China/Tib/07 |
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| LIU Wen-hua,,BAO Jing-yue,WU Xiao-dong,WANG Zhi-liang.Genome Sequencing and Analysis of a Peste Des Petits Ruminants Virus Isolate,China/Tib/07[J].Chinese Journal of Virology,2010(4). |
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| Authors: | LIU Wen-hua BAO Jing-yue WU Xiao-dong WANG Zhi-liang |
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| Institution: | LIU Wen-hua1,2,3,BAO Jing-yue2,WU Xiao-dong2,WANG Zhi-liang2(1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China,2.National Diagnostic Center for Exotic Animal Diseases,China Animal Health and Epidemiology Center,Qingdao 266032,3.Animal Science and Veterinary Medicine College,Qingdao Agricultural University,Qingdao 266109,China) |
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| Abstract: | Peste des petits ruminants virus is a member of Morbillivirus Paramyxoviridae.The complete genome of a Peste des petits ruminants virus(PPRV)isolate,China/Tib/07 was sequenced and molecular characteristics was analyzed.The internal sequences of the virus genome were amplified by RT-PCR with primers designed according to the published data in GenBank,while the sequences of the 3' and 5' ends of the genome were determined by RACE.Amplification products were directly sequenced,assembled and analyzed with DNASt... |
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| Keywords: | Peste des petits ruminants virus morbillivirus coding region complete genome nucleotide sequence |
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