| 不同病变型J亚群禽白血病病毒LTR启动子序列分析及活性比较 |
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| 引用本文： | 张贺楠,齐岩,史伟伟,梁艺瑜,刘红波,曹伟胜,廖明. 不同病变型J亚群禽白血病病毒LTR启动子序列分析及活性比较[J]. 病毒学报, 2010, 26(5): 402-406 |
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| 作者姓名： | 张贺楠 齐岩 史伟伟 梁艺瑜 刘红波 曹伟胜 廖明 |
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| 作者单位： | 华南农业大学,兽医学院,农业部动物疫病防控重点开放实验室,广州,510642;华南农业大学,兽医学院,农业部动物疫病防控重点开放实验室,广州,510642;中国牧工商(集团)总公司,北京,100070 |
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| 基金项目： | 国家自然科学基金(30771612); NSFC-广东联合基金(U0831002); 广东省自然科学基金(8151064201000065); 广东省科技计划项目(2009A020101006); 肉鸡产业技术体系项目(nycytx-42-G3-03) |
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| 摘 要： | 从ALV-J中国地方分离株SCAU-HN06株(血管瘤病变型)、NX0101株和JS-nt株(骨髓瘤病变型)病毒的细胞培养物提取前病毒DNA,通过PCR扩增各毒株的LTR并克隆,随后进行测序分析。与国内外ALV-J参考毒株LTR序列比较发现:国内地方分离株与英国ALV-J原型株HPRS-103和美国ALV-J原型株ADOL-7501的LTR核苷酸序列相似性为88.0%~97.2%;LTR中的U5区及R区具有较高的保守性,而U3区内存在较大差异。将不同病变型ALV-J的LTR片段分别插入pCAT-basic载体CAT报告基因5'端。用所得的重组报告基因表达质粒转染DF-1细胞,48h后通过测定转染细胞中的CAT表达量来评价LTR启动子的活性。结果表明,SCAU-HN06株与骨髓瘤病变型ALV-J(JS-nt株,NX0101株)LTR启动子活性差异不显著。
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| 关 键 词： | J亚群禽白血病病毒 血管瘤 长末端重复序列 启动子活性 |
Sequence and promoter efficacy analysis of avian leukosis virus subgroup J strains of different pathotypes |
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| Zhang He-nan,Qi Yan,Shi Wei-wei,Liang Yi-yu,Liu Hong-bo,Cao Wei-sheng,Liao Ming. Sequence and promoter efficacy analysis of avian leukosis virus subgroup J strains of different pathotypes[J]. Chinese journal of virology, 2010, 26(5): 402-406 |
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| Authors: | Zhang He-nan Qi Yan Shi Wei-wei Liang Yi-yu Liu Hong-bo Cao Wei-sheng Liao Ming |
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| Affiliation: | ZHANG He-nan1,QI Yan1,2,SHI Wei-wei1,LIANG Yi-yu1,LIU Hong-bo1,CAO Wei-sheng1,LIAO Ming1(1.College of Veterinary Medicine,South China Agricultural University,Key Laboratory of Animal Disease Control and Prevention,Ministry of Agriculture,Guang zhou 510642,China,2.China Animal Husbandery Group,Beijing 100070,China) |
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| Abstract: | To characterize the long terminal repeat(LTR) of the ALV-J strain which can induce hemangioma,fragments of provirus LTR of the three different ALV-J strains SCAU-HN06,NX0101 and JS-nt were amplified with a pair of specific primers,then cloned and subjected to sequence analysis.In comparison with the prototype ALV-J strains HPRS-103 and ADOL-7501,the LTRs of domestic strains(SCAU-HN06,NX0101,JS-nt and SD07lk1) had an 88.0%-97.2% nucleotide sequence identity;the U5 and R regions in the LTR had a high nucleoti... |
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| Keywords: | avian leukosis virus subgroup J hemangioma long terminal repeat promoting efficacy |
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