| RNA干扰抑制登革病毒复制的研究 |
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| 引用本文： | 岳锦亚,吴新伟,伍业健,李向忠,蒋力云,李巧艳,李磊,杨霞. RNA干扰抑制登革病毒复制的研究[J]. 病毒学报, 2010, 26(5): 373-378 |
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| 作者姓名： | 岳锦亚 吴新伟 伍业健 李向忠 蒋力云 李巧艳 李磊 杨霞 |
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| 作者单位： | 广州市疾病预防控制中心,广州,510080;中山大学,中山医学院,广州,510080;广州市疾病预防控制中心,广州,510080;中山大学,中山医学院,广州,510080 |
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| 基金项目： | 广东省科技计划项目(2008B030303041); 广州市科技计划项目(2006J1-C0141,2008J1-C191,2008Z1-E231); 广州市医学科学技术研究重点项目(2006-ZDi-10,2008-ZDi-12) |
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| 摘 要： | 为了研究RNA干扰(RNAi)对Ⅰ型登革病毒(DENV-1)在白纹伊蚊C6/36细胞内复制的影响,本研究设计并合成针对I型登革病毒Pr M基因的小干扰RNA,以脂质体法转染入C6/36细胞后,用DENV-1感染已转染的细胞,观察细胞病变效应,MTT法检测细胞存活率,荧光定量RT-PCR检测登革病毒RNA含量。结果表明:转染siRNA的C6/36细胞在受登革病毒攻击7天后仍无明显细胞病变效应,细胞存活率比对照组提高2.26倍,细胞内登革病毒RNA拷贝数比对照组降低约97.54%。说明利用RNA干扰技术能有效抑制登革病毒核酸在C6/36细胞内复制,并对细胞具有一定保护作用,为登革热的防治提供了新的思路。
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| 关 键 词： | 登革病毒 C6/36细胞 RNA干扰 荧光定量PCR |
Study on the inhibitory effect of RNA interference on replication of dengue virus |
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| Yue Jin-ya,Wu Xin-wei,Wu Ye-jian,Li Xiang-zhong,Jiang Li-yun,Li Qiao-yan,Li Lei,Yang Xia. Study on the inhibitory effect of RNA interference on replication of dengue virus[J]. Chinese journal of virology, 2010, 26(5): 373-378 |
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| Authors: | Yue Jin-ya Wu Xin-wei Wu Ye-jian Li Xiang-zhong Jiang Li-yun Li Qiao-yan Li Lei Yang Xia |
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| Affiliation: | YUE Jin-ya1,2,WU Xin-wei1,WU Ye-jian1,LI Xiang-zhong1,JIANG Li-yun1,LI Qiao-yan2,LI Lei2,YANG Xia2(1.Guangzhou Center for Disease Control and Prevention,Guangzhou 510080,China,2.Department of Biochemistry,Zhongshan Medical School,Sun Yat-sen University,China) |
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| Abstract: | To investigate the inhibitory effect of RNA interference (RNAi) on dengue virus I (DENV-1) replication. Small interfering RNA (siRNA) against the PreM gene of dengue virus was synthesized and transfected into C6/36 cells with liposome, which was then attacked by DENV-1 virus. The antiviral effect of siRNA was evaluated by cytopathic effect (CPE), the cell survival rate measured by MTT, and virus RNA quantified by real-time RT-PCR. The results showed that after 7 days post infection of dengue virus, the transfected C6/36 cells showed less CPE. The cell survival rate of the transfected C6/36 cells increased by 2.26 fold, and the amount of virus RNA in the transfected cells was reduced by about 97.54% as well. These findings indicated that the siRNA could effectively inhibit dengue virus RNA replication, and protect C6/36 cells from viral attack, indicating its potential role in prevention and treatment of dengue fever. |
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| Keywords: | dengue virus C6/36 cell RNA interference real-time PCR |
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