Involvement of cAMP-Dependent protein kinase and intracellular free calcium ions in regulation of ovulation of the follicle-enclosed oocytes ofRana temporaria by gonadotropic hormones |
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Authors: | M N Skoblina |
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Institution: | (1) Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, 117808 Moscow, Russia |
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Abstract: | The inhibitor of cAMP-dependent protein kinase N-2-(methylamino)ethyl]-5-isoquinoline-sulfonamide (H-8) (12.5–50 μM) decreased
the rate of ovulation of the follicle-enclosedRana temporaria oocytes induced by the homologous pituitary extract in amphibian Ringer solution and in a chloride-free medium. The inhibitor
of voltage-dependent calcium channels diltiazem (10 and 100 μM) decreased the rate of ovulation in Ringer solution but did
not affect it in a chloride-free medium or decreased the ovulation inhibitory effect of this medium. It was concluded that
cAMP-dependent protein kinase and intracellular free calcium ions were involved as second messengers in the gonadotropin regulation
not only in maturation of amphibian oocytes but also in ovulation. |
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Keywords: | cAMP-dependent protein kinase A intracellular concentration of free calcium ions H-8 diltiazem follicle-enclosed oocyte maturation ovulation amphibians |
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