Translational features of human alpha 2b interferon production in Escherichia coli |
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| Authors: | Valente C A Prazeres D M F Cabral J M S Monteiro G A |
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| Institution: | Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. |
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| Abstract: | The yield of human alpha 2b interferon in Escherichia coli was optimized by replacement of low-usage arginine codons located in the mRNA 5' end. The differences observed among the various gene variants suggest that codon usage, Shine-Dalgarno-like sequences, and mRNA secondary structure contribute to the performance of E. coli translation machinery. |
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