Pectic polysaccharides in carrot cells growing in suspension culture |
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Authors: | Tetsuya Asamizu Noriko Nakayama Arasuke Nishi |
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Institution: | (1) Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 930-01 Sugitani, Toyama, Japan |
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Abstract: | Pectic polysaccharides in the cell wall of suspension-cultured carrot cells (Daucus carota L.) were fractionated into high- and low-molecular-weight components by molecular-sieve chromatography with a Sepharose 4B column. During the phase of cell-wall expansion, the relative content of low-molecular-weight polymers rapidly increased. Electrophoretic analyses of these fractions showed that the high-molecular-weight components were largely composed of neutral and weakly acidic polymers while the low-molecular-weight fraction contained, in addition to neutral polymers, strongly acidic polyuronides in which the content of neutral sugars was very small. The accumulation of a large amount of the strongly acidic polyuronides occurred in a late stage of cell-wall growth, concomitant with a marked decrease in the high-molecular-weight components.Abbreviation MW
molecular weight |
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Keywords: | Cell culture (wall composition) Cell wall (composition) Daucus Pectic polysaccharides |
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