Several phenotypic changes in the cell envelope of Agrobacterium tumefaciens chvB mutants are prevented by calcium limitation |
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Authors: | Saskia Swart Trudy J J Logman Ben J J Lugtenberg Gerrit Smit Jan W Kijne |
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Institution: | (1) Institute of Molecular Plant Sciences, Leiden University, P.O. Box 9505, 2333 AL Leiden, The Netherlands;(2) Mogen International, Einsteinweg 97, 2333 CB Leiden, The Netherlands |
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Abstract: | The chvB gene of Agrobacterium tumefaciens encodes a 235 kDa proteinaceous intermediate involved in the synthesis of -1,2-glucan. chvB mutants show a pleiotropic phenotype. Besides not to produce cyclic -1,2-glucan, chvB mutants have been reported to be avirulent, attachment-deficient, and nonmotile. In this study we report additional differences from the parent strain, probably all linked to changes in the cell envelope. This pleiotropic phenotype — except for attachment and virulence — could largely be prevented by growing chvB cells with low levels of calcium. Although a role for -1,2-glucan in osmoadaptation has been proposed, the mode of action of -1,2-glucan is not known. We speculate that in A. tumefaciens -1,2-glucan stabilizes membranes, which would be important especially in hypotonic media containing calcium.Abbreviations Cb
carbenicillin
- Km
kanamycin
- TCA
trichloroacetic acid
- Kav
fraction of the stationary gel volume available for diffusion
- LPS
lipopolysaccharide
- SDS-PAGE
Sodium dodecyl sulphate polyacrylamide gel electrophoresis |
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Keywords: | Agrobacterium -1" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-1 2-Glucan chvB Mutants— Ca2+ |
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