Protoplast preparation and regeneration in Nocardia mediterranei |
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Authors: | T. Schupp M. Divers |
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Affiliation: | CIBA-Geigy Ltd., Department of Biotechnology, K 681, CH-4002 Basle, Switzerland |
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Abstract: | Abstract A standard procedure for the preparation of viable Streptomyces protoplasts was found to be unsuccessful for the rifamycin-producing actinomycete Nocardia mediterranei . An alternative method for N. mediterranei protoplast preparation is described. This method was also found to be applicable to several other ansamycin-producing actinomycetes. |
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Keywords: | Nocardia mediterranei protoplast preparation rifamycin ansamycin |
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