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Protoplast preparation and regeneration in Nocardia mediterranei
Authors:T Schupp  M Divers
Institution:CIBA-Geigy Ltd., Department of Biotechnology, K 681, CH-4002 Basle, Switzerland
Abstract:Abstract A standard procedure for the preparation of viable Streptomyces protoplasts was found to be unsuccessful for the rifamycin-producing actinomycete Nocardia mediterranei . An alternative method for N. mediterranei protoplast preparation is described. This method was also found to be applicable to several other ansamycin-producing actinomycetes.
Keywords:Nocardia mediterranei            protoplast preparation  rifamycin  ansamycin
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