Regulation of abscisic acid metabolism: towards a metabolic basis for abscisic acid-cytokinin antagonism |
| |
Authors: | Cowan A; Cairns A; Bartels-Rahm B |
| |
Institution: | Department of Horticultural Science, Department of Agronomy, Department of Chemistry and Technology, University of Natal, Pietermaritzburg, Private Bax X01, Scottsville 3209, South Africa; Corresponding author; Fax: +27 331 2605073; E-mail: cowan@hort.unp.ac.za |
| |
Abstract: | The penultimate step in abscisic acid (ABA) biosynthesis involves oxidation
of xanthoxal (XAN) catalysed by a molybdenum-cofactor (MoCo)-containing
aldehyde oxidase (AO) and represents one potential site of regulation of
ABA in plant tissues. In an attempt to understand the biochemical basis for
cytokinin-abscisic acid (CK-ABA) antagonism the effect of several CKs,
molybdate, tungstate and allopurinol (an inhibitor of xanthine oxidase
activity and purine metabolism) on the formation of XAN, ABA and related
catabolites in mesocarp of ripening avocado (Persea
americana Mill. cv. Hass) was investigated. Treatment with
either adenine (Ade), isopentenyladenine (2iP) or zeatin (Z) enhanced
conversion of ABA to phaseic acid (PA) and caused a reduction in the amount
of radioactivity incorporated from
3R-2-14C] mevalonolactone
(MVL) into ABA by stimulating overall ABA metabolism. Ancymidol and
N-(2-chloro-4-pyridyl)-N-phenylur
ea (CPPU), while not affecting formation of PA and DPA, appeared to retard
ABA biosynthesis which resulted in the accumulation of XAN. Tungstate
caused accumulation of XAN at the expense of ABA and related acidic
metabolites while molybdate and allopurinol accelerated ABA metabolism,
i.e. formation of XAN, ABA, PA, and DPA. These findings are discussed in
terms of the regulation of the ABA biosynthetic pathway in avocado fruit by
CK-induced suppression of xanthine dehydrogenase (XDH) activity and a model
illustrating the proposed metabolic interrelationship is
presented. |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|