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HPLC-EC analysis of catechols and indoles in rat brain dialysates
Authors:T Sharp  T Zetterstr?m  H G Series  A Carlsson  D G Grahame-Smith  U Ungerstedt
Institution:1. Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas – SBFis/UNESP, Brazil;2. Laboratory of Neuroendocrinology, School of Dentistry of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil;3. Laboratory of Endocrine Physiology and Aging, Araçatuba Dental School, Universidade Estadual Paulista, Araçatuba, SP, Brazil;1. Department of Radiology, West China Hospital, Sichuan University, Chengdu, China;2. Sichuan Primed Bio-Tech Group Co., Ltd., Chengdu, China;3. Department of Anesthesiology and Institute of Neurological Disease, Translation Neuroscience Center, West China Hospital, Sichuan University, Chengdu, China;4. Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO, USA;1. Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China;2. Division of Neurosurgery, Department of Surgery, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong;3. Department of Neurology, Affiliated Hangzhou First People''s Hospital, Zhejiang University School of Medicine, Hangzhou, China;4. Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China;5. The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Southern Medical University, Guangzhou, China;6. Brain Research Institute, Zhejiang University, Hangzhou, China;7. Collaborative Innovation Center for Brain Science, Zhejiang University, Hangzhou, China
Abstract:This short article reviews HPLC-EC methodology that we are currently applying to measure DA, 5-HT and their acid metabolites in rat brain dialysates collected in vivo. HPLC-EC systems based on standard-bore HPLC columns are described which are sufficiently sensitive to allow detection of the monoamine transmitters and their metabolites in regional brain dialysates collected at 10-20 min intervals. A large reduction in sample requirement was achieved by "down-scaling" the conventional HPLC-EC assay to incorporate small-bore HPLC columns. The small-bore systems allowed monoamines to be detected in samples collected over 1 to a few minutes.
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