Comparison of different probe-level analysis techniques for oligonucleotide microarrays |
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Authors: | Rosati Barbara Grau Frederic Kuehler Anneke Rodriguez Samantha McKinnon David |
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Institution: | State University of New York at Stony Brook, Stony Brook, NY, USA. |
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Abstract: | Three different software packages for the probe-level analysis of high-density oligonucleotide microarray data were compared using an experiment-derived data set that was validated using real-time PCR. The efficiency with which these three programs could identify true positives in this data set was assessed. In addition, estimates of false-positive and false-negative rates were determined. The performance of the programs using very small data sets was also compared, and recommendations for use are suggested. |
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