Agrobacterium-mediated transformation and regeneration of guayule |
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Authors: | Zhiqiang Pan Jingkun Ho Qin Feng Dao-Shun Huang Ralph A. Backhaus |
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Affiliation: | (1) Department of Botany, Arizona State University, Box 871601, 85287-1601 Tempe, AZ, U.S.A.;(2) Department of Biological Sciences, Shihezi Agricultural College, Shihezi, Xinjiang, P.R. China;(3) Department of Tropical Crops, South China College of Tropical Crops, Hainan, P.R. China |
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Abstract: | In vitro grown shoot tissue of facultative apomictic lines of guayule (Parthenium argentatum Gray), a rubber producing desert shrub, were transformed by Agrobacterium-mediated DNA transfer and regenerated into complete plants. Guayule shoots of lines 11591, UC101 and UC104 were inoculated with A. tumefaciens strains LBA4404 or PC2760 harboring the binary vector pCGN1557. Axillary shoots were regenerated from transformed cells and rooted in vitro in the presence of kanamycin. Genetic transformation in all cases was verified by Southern blot analysis. Transgenic plants were grown to maturity in the greenhouse and, as predicted for apomictic species, all seed produced possessed kanamycin resistance. Because apomicts have limitations for gene transfer by normal sexual crosses, this method offers a new means of transferring genes into this species.Abbreviations BA benzyladenine - EDTA ethylene diamine tetraacetate - kanR kanamycin resistance - MS salts salts of Murashige and Skoog medium (1962) - NAA naphthalene acetic acid - NPT-II neomycin phosphotransferase - SDS sodium dodecyl sulfate |
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Keywords: | apomixis rubber biosynthesis transgenic guayule Parthenium argentatum |
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