| Affiliation: | (1) Japan Grassland Farming and Forage Seed Association, Forage Crop Research Institute, 388-5, Higashiakada, Nishinasuno, Tochigi 329-2742, Japan;(2) Hokkaido Kitami Agricultural Experiment Station, Kunneppu-cho, Tokoro-gun, Hokkaido 099-1496, Japan |
| Abstract: | To develop simple sequence repeat (SSR) markers for the hexaploid forage grass timothy (Phleum pratense L.), we used four SSR-enriched genomic libraries to isolate 1,331 SSR-containing clones. All four libraries contained a high percentage of perfect clones, ranging from 78.1% to 91.6%. From these clones, we developed 355 SSR markers when tested from 502 SSR primer pairs. Using all 355 SSR markers we tested one screening panel consisting of eight timothy clones to detect the level of polymorphism and identify a set of loci suitable for framework mapping. The SSR markers detected 90.4% polymorphism between the parents of a pseudo-testcross F1 population. These SSR markers will provide an ideal marker system to assist with gene targeting, QTL (quantitative trait locus) mapping, and marker-assisted selection in timothy.Electronic Supplementary Material Supplementary material is available in the online version of this article at |
