The spatial relationship of the viral and H-2 antigens recognized by anti-viral CTLs |
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Authors: | Arthur H Hale |
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Institution: | (1) Department of Microbiology and Immunology, Bowman Gray School of Medicine, 27103 Winston-Salem, North Carolina |
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Abstract: | With the use of monospecific rabbit anti-G protein and mouse monoclonal anti-H-2Kk, we have analyzed the spatial relationship of the serologically defined H-2Kk antigens and the major surface glycoprotein (G protein) of vesicular stomatitis virus (VSV) to those antigens recognized by B10.A (k, d) anti-VSV cytotoxic T lymphocytes (CTLs). The ability of monoclonal anti-H-2Kk or rabbit anti-G protein to inhibit specifically the cytolytic activity of B10.A anti-VSV CTLs indicates that the G protein and the H-2Kk molecules are in close proximity to the viral and H-2Kk antigens recognized by the anti-VSV (CTLs. By the method of sequential immunoprecipitation, we also demonstrated that only 10–30 percent of the serologically defined G and H-2Kk molecules are in theG-H-2K
k
complexes.Abbreviations used in this paper Con A
Concanavalin A
- cpm
counts per minure
- CTLs
cytotoxic T lymphocytes
- E: T ratio
effector: target ratio
- G
major surface glycoprotein of VSV
- MHC
major histocompatibility complex
- MOI
multiplicity of infection
- NP40
Nonidet-P40
- PAGE
polyacrylamide gel electrophoresis
- PBS
phosphate-buffered saline
- SaCI
Staphylococcus aureus, Cowan I strain
- SDS
sodium dodecyl sulfate
- UV
ultraviolet light |
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Keywords: | |
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