The instantly released Drosophila immune proteome is infection-specific |
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Authors: | Vierstraete Evy Verleyen Peter Sas Filip Van den Bergh Gert De Loof Arnold Arckens Lutgarde Schoofs Liliane |
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Affiliation: | Laboratory of Developmental Physiology, Genomics and Proteomics, K.U.Leuven, Naamsestraat 59, B-3000 Louvain, Belgium. evy.vierstraete@bio.kuleuven.ac.be |
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Abstract: | In this study, we analyzed the hemolymph proteome of Drosophila third instar larvae, which were induced with a suspension of Gram-positive bacteria or yeast. Profiling of the hemolymph proteins of infected versus non-infected larvae was performed by two-dimensional difference gel electrophoresis. Infection with Micrococcus luteus or Saccharomyces cerevisiae induced, respectively, 20 and 19 differential protein spots. The majority of the spots are specifically regulated by one pathogen, whereas only a few spots correspond to proteins altered in all cases of challenging (including after challenge with lipopolysaccharides). All of the upregulated proteins can be assigned to specific aspects of the immune system, as they did not increase in the hemolymph of sterile pricked larvae. Next to known immune proteins, unannotated proteins were identified such as CG4306 protein, which has homologues with unknown function in all metazoan genome databases available today. |
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Keywords: | Two-dimensional difference gel electrophoresis Secretome Immune response proteins Drosophila melanogaster Micrococcus luteus Saccharomyces cerevisiae |
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