| Abstract: | A ramp voltage clamp measurement described previously is used to detect alterations in the frog skin current-potential (I-V) characteristic following removal or replacement of various ions in the solutions bathing the skin. The ionic requirements for the maintenance of a negative-slope I-V property are the following: Ca++, Na+, and Cl- must be in the outside solution; K+ and Cl- must be in the inside solution. Removal of any one of these ions from its respective solution results in the decay and eventual disappearance of the negative slope. The similarity between the I-V characteristic following Ca++ removal with EDTA from the outside solution and the I-V relation in a refractory skin suggests that the loss (refractory state) and recovery of the negative slope is a consequence of unbinding and subsequent rebinding of Ca++ to membrane sites. The role of the univalent ions is not clear—presumably some or all of these ions constitute the current through the skin; however, some of these ions may also be involved in maintaining a membrane condition necessary for the existence of a negative slope I-V relation. Further, excitation does not appear to be a direct consequence of the Na+ pump. |
