Genetic and phytochemical diversities of Cynomorium songaricum Rupr. in Northwest China indicated by ISSR markers and HPLC-fingerprinting |
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| Institution: | 1. Science and Technology Division, Okinawa National College of Technology, 905 Henoko, Nago, Okinawa 905-2192, Japan;2. Makino Herbarium, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 192-0397, Japan;1. SDM Centre for Research in Ayurveda and Allied Sciences, Laxminarayana Nagar, Kuthpady, Udupi 574118, India;2. Krupanidhi Degree College, Bangalore 560035, India;3. Department of Botany and Microbiology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia;1. Central Department of Botany, Tribhuvan University, Kathmandu, Nepal;2. Annapurna Research Centre, Kathmandu, Nepal |
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| Abstract: | Cynomorium songaricum Rupr. is a parasitic plant and its most common host is Nitraria tangutorum Bobr. which grows in desert areas. To support the sustainable use and conservation of C. songaricum, genetic and phytochemical studies were undertaken on 16 different populations. Samples were analyzed using inter-simple sequence repeat (ISSR) markers and high performance liquid chromatography (HPLC)-fingerprinting. Total phenolic acids were determined by UV spectrophotometry. At the population level, percentage of polymorphic bands (PPB) ranged from 29.89 to 64.94%, Nei's genetic diversity index (H) ranged from 0.0931 to 0.2055, Shannon's information index (I) ranged from 0.1422 to 0.3140. 16 populations were classified into four chemotypes according to HPLC-fingerprinting analysis. Total phenolic acids contents ranged from 56.79 to 123.50 mg g?1. The results of the genetic and chemical studies are used to suggest a planting and conservation system for C. songaricum. |
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