Isolation and characterization of fifteen polymorphic microsatellite loci in the threatened freshwater mussel Solenaia oleivora (Bivalvia: Unionidae) |
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| Affiliation: | 1. Key Laboratory of Freshwater Animal Breeding of the Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China;2. Hubei Key Laboratory of Wetland Evolution and Ecological Restoration, China University of Geosciences, Wuhan 430074, PR China;1. Harvard School of Public Health, Department of Epidemiology, Harvard University, Boston, MA, USA;2. Global Biotech Consulting Group (GBC Group), Mexico City, Mexico;3. Organization for Economic Co-operation and Development (OECD), Directorate for Science, Technology and Innovation, Paris, France;1. School of Materials Science and Engineering, State Key Laboratory of Material Processing and Die & Mould Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China;2. Research Laboratory of Hydrothermal Chemistry, Faculty of Science, Kochi University, Kochi 780-8520, Japan;3. Green Innovative Chemistry Research Unit, Department of Chemistry, Thammasat University, Pathumthani 12120, Thailand;1. Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, PR China;2. Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, PR China;1. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, 7602 Stellenbosch, South Africa;2. Post-Harvest and Wine Technology Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa;1. Key Laboratory of Ministry of Water Resources for Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem, Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, 430079, China;2. Hubei Provincial Collaborative Innovation Center for Water Resources Security, Wuhan 430072, China |
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| Abstract: | Freshwater mussels are among the most endangered fauna in the world, while little is known about their genetic diversity. We isolated 15 polymorphic microsatellite loci in a threatened freshwater mussel, Solenaia oleivora. The characteristics of these loci were tested with 46 individuals. The number of alleles per locus ranged from 2 to 15, and the PIC value ranged from 0.250 to 0.880. The observed and expected heterozygosity ranged from 0.167 to 0.929 and from 0.297 to 0.900, with an average of 0.562 and 0.647, respectively. Eight loci deviated significantly from Hardy–Weinberg equilibrium. These polymorphic microsatellites will be useful for the assessment of population genetic diversity and resource conservation of S. oleivora. Cross-species amplification demonstrated that six of 15 microsatellite loci amplified successfully in two other unionid species. |
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