Characterization of chloroplast microsatellite loci from whole chloroplast genome of Camellia taliensis and their utilization for evaluating genetic diversity of Camellia reticulata (Theaceae) |
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| Institution: | 1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, The Chinese Academy of Sciences, Kunming 650201, China;2. Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, The Chinese Academy of Science, Kunming 650201, China;3. University of the Chinese Academy of Sciences, Beijing 100039, China;1. College of Life Sciences, Shaanxi Normal University, Xi''an 710062, China;2. National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Xi''an, Shaanxi 710062, China;3. Meteorological Bureau of Shaanxi Province, Xi''an 710014, China;1. National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi''an, 710062, China;2. Core Research Laboratory, the Second Affiliated Hospital, School of Medicine, Xi''an Jiaotong University, Xi''an, 710004, China;3. Climate Research Center, Meteorological Bureau of Shaanxi Province, Xi''an, 710014, China;1. College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China;2. National Flower Engineering Research Centre, Key Laboratory for the Genetics and Breeding of Forest Trees and Ornamental Plants, Beijing 100083, China;3. Forestry Department of Shaanxi Province, Xian 710082, China |
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| Abstract: | This study characterized chloroplast microsatellite markers for Camellia reticulata, a famous ornamental and edible economic tree species only distributed in Southwestern China. Thirty-two chloroplast microsatellite markers were originally annotated in the whole chloroplast genome of Camellia taliensis, ten polymorphic microsatellite markers were tested in 96 individuals from four natural populations of C. reticulata. Alleles numbered from two to four, and average value of Shannon's Information index, Nei's gene diversity, total genetic diversity, genetic diversity within populations, gene differentiation coefficient and gene flow index were 0.408, 0.225, 0.217, 0.102, 0.530 and 0.443, respectively. Our results showed high genetic differentiation and limited gene flow among the studied populations, which may be explained by recent fragmentation of the remnant populations due to human destruction and disturbance of natural habitats of the species. The identified cpSSR markers will be useful for the future studies on population genetics, conservation biology and phylogeography of C. reticulata. |
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| Keywords: | Theaceae Polymorphisms Population genetics cpSSR Chloroplast microsatellite |
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