Genetic diversity of castor bean (Ricinus communis L.) in Northeast China revealed by ISSR markers |
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| Institution: | 1. College of Agriculture, Inner Mongolia University for the Nationalities, Tongliao 028043, China;2. Tongliao Academy of Agricultural Science, Inner Mongolia, Tongliao 028015, China;3. College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China;4. Inner Mongolia Industrial Engineering Research Center of Universities for Castor, Tongliao 028043, China;1. School of Computer Science & Communications Engineering, Jiangsu University, 301, Xuefu Road, Jingkou District, Zhenjiang, Jiangsu 212013, PR China;2. School of Computer Science & Technology, Nanjing University of Science and Technology, 200, Xiaolingwei, Xuanwu District, Nanjing, Jiangsu 210094, PR China;3. Jiangsu Jingjiang People''s Hospital & The Affiliated Hospital with Medical School of Yangzhou University, 28, Zhongzhou Road, Jingjiang, Jiangsu 214500, PR China;1. Laboratoire des RadiosIsotopes, Université of Antananarivo, BP 3383, 101, Antananarivo, Madagascar;2. Eszterházy Károly University, Eger, Hungary;3. Department of Biology, University of Iowa, Iowa City, IA, 52240 USA;4. IRD, UMR Eco&Sols, Laboratoire des RadiosIsotopes, Université of Antananarivo, BP 3383, 101, Antananarivo, Madagascar;1. KU Leuven, Faculty of Psychology and Educational Sciences, Research group Experimental Psychology, Laboratory for Perceptual Dynamics, Tiensestraat 102, 3000 Leuven, Belgium;2. KU Leuven, Department of Computer Science, Celestijnenlaan 200A, 3001 Heverlee, Belgium;3. Queen Mary University of London, School of Engineering and Materials Science, Mile End Road, London E1 4NS, UK;4. Eindhoven University of Technology, Department of Mechanical Engineering, Dynamics and Control group, P.O.Box 513, 5600 MB Eindhoven, The Netherlands;1. Department of Chemistry, Memorial University of Newfoundland, St. John''s, Newfoundland, Canada A1B 3X7;2. Physical Chemistry Department, National Research Centre, El Buhouth St., Dokki, Cairo 12311, Egypt |
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| Abstract: | Inter-Simple Sequence Repeat (ISSR) markers were employed to analyze the genetic diversity of Ricinus communis L. in northeastern China plants. We selected ten primers that produced clear, reproducible and multiple bands for these experiments and 179 bands were obtained across 39 genotypes. Polymorphic band ratios ranged from 100% to a minimum of 78.9% with an average of 96.4% while band numbers were comprised between 13 (UBC823) and 23 (UBC856). The results obtained from UPGMA clustering dendrogram and PCoA lead to 39 distinct castor bean accessions belonging to four major groups. We found that all groups shared a common node with 66% similarity while Jaccard's similarity coefficient ranged from 0.58 to 0.92. Compatible inference was also observed from the high values of heterozygosity (Ht = 0.3378 ± 0.0218), Nei's genetic diversity (H = 0.1765 ± 0.2090), and Shannon's information index (I = 0.4942 ± 0.1872). In addition, our data reveal a Nei's genetic differentiation index (GST) of 0.3452 and estimated the gene flow (Nm) at 0.9482. These findings clearly suggest a genetic diversity in castor bean germplasms from various geographic origins and contribute to our understanding of breeding and conservation of castor beans. |
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| Keywords: | Genetic diversity ISSR Gene flow |
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