Estimation of genetic diversity in some Iranian cornelian cherries (Cornus mas L.) accessions using ISSR markers |
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| Affiliation: | 1. Faculty of Natural Sciences and Institute for Science & Technology in Medicine, Keele University, Staffordshire ST5 5BG, UK;2. Life Sciences Group, Institut Laue-Langevin, 71 avenue des Martyrs, 38000 Grenoble, France;3. Wits Research Institute for Malaria (WRIM), Faculty of Health Sciences, University of the Witwatersrand, National Health Laboratory Service, Johannesburg, South Africa;4. Univ. Grenoble Alpes, IBS, F-38044 Grenoble, France;5. CNRS, IBS, F-38044 Grenoble, France;6. CEA, DSV, IBS, F-38044 Grenoble, France;7. ESRF, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France;1. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, No. 5, Xinmofan Road, Nanjing 210009, China;2. College of Biotechnology and Pharmaceutical Engineering, National Engineering Technique Research Center for Biotechnology, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing 211816, China;3. Department of Chemical & Biomolecular Engineering, The University of Akron, Akron, OH 44325, USA |
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| Abstract: | Iran is an important producer of cornelian cherries (Cornus mas L.). Seed propagation and long term human selection have given rise to a great diversity of trees. In this study, ISSR marker was used for genetic diversity evaluation of 40 accessions of cornelian cherries (C. mas L.). With 20 ISSR primers, 171 polymorphic bands were detected with polymorphism ratio range of 80–100%. The average polymorphism information content (PIC) was 0.46, which shows that the majority of primers are informative. The average values of Effective Multiplex Ratio (EMR), Marker Index (MI), effective number of alleles (Ne), Nei's gene diversity (He) and Shannon's information index (I) for all primers were 8.0, 3.75, 1.756, 0.416 and 0.595 respectively. Based on these results, ISSR analysis can be used for the characterization and grouping of cornelian cherry accessions. This is the first study demonstrating that ISSR analyses can be used to differentiate and classify cornelian cherry accessions. |
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