Genetic diversity of wild Auricularia auricula-judae revealed by ISSR analysis |
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| Institution: | 1. The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;2. Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Science, Beijing 100700, China;3. School of Life Sciences, Peking University, Beijing 100871, China;4. Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435, China;1. Institute of Vegetable Crops, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China;3. Faculty of Agriculture and Environmental Sciences, Chuka University, P.O. Box 109-60400, Chuka, Kenya |
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| Abstract: | Auricularia auricula-judae is an edible and medicinal jelly mushroom, and it is widely cultivated in China. In the present study, inter-simple sequence repeat (ISSR) molecular marker was employed to assess the genetic diversity of 22 wild strains and two cultivars of A. auricula-judae obtained from many ecological regions of China. With the use of 11 ISSR primers, a total of 368 (99.7%) and 81 (71.7%) polymorphic loci in wild and cultivated strains were detected, respectively. The mean genetic similarity of A. auricula-judae was 0.75, based on the dendrogram generated via similarity coefficient, all the tested strains were classified into six groups at a similarity level of 76%, and the two cultivated strains consisted of one group alone. The results of principal coordinate analysis were in accordance with UPGMA clustering, and the first most informative coordinate accounted for 75.9% of the variations, indicated a high level of genetic diversity among wild A. auricula-judae strains. In general, ISSR marker was an effective method to discriminate the A. auricula-judae stains and to evaluate their genetic diversity. |
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