Genetic diversity in Mentha cervina based on morphological traits,essential oils profile and ISSRs markers |
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| Affiliation: | 1. Centro de Botânica Aplicada à Agricultura (CBAA), Instituto Superior de Agronomia, Technical University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal;2. Centro Interdisciplinar de Investigação e Inovação (C3i), Escola Superior Agrária de Elvas, Instituto Politécnico de Portalegre, Edifício do Trem Auto, Avenida 14 de Janeiro, 7350-903 Elvas, Portugal;3. Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Av. Transnordestina s.n., 44036-900 Feira de Santana, Bahia, Brazil;4. Universidade de Lisboa, Faculdade de Ciências de Lisboa, Departamento de Biologia Vegetal, IBB, Centro de Biotecnologia Vegetal, C2, Piso 1, Campo Grande, 1749-016 Lisboa, Portugal;5. Centro de Engenharia dos Biossistemas (CEER), Instituto Superior de Agronomia, Technical University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal;1. UMR408 Sécurité et Qualité des Produits d’Origine Végétale, INRA, F-84000 Avignon, France;2. UMR408 Sécurité et Qualité des Produits d’Origine Végétale, Université d’Avignon et des Pays du Vaucluse, F-84000 Avignon, France;1. Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore;2. Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A⁎STAR), Singapore 138673, Singapore;3. Division of Microbiology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan;1. Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia;2. Department of Botany, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia;3. Present Address: Department of Epizootiology and Parasitology, Institute of Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181, Košice, Slovakia;1. Department of Horticultural Sciences, Faculty of Agriculture Sciences and Engineering, University of Tehran, Karaj, 31587, Iran;2. Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran |
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| Abstract: | Morphological, phytochemical and genetic differences were studied to evaluate the level and distribution of diversity in twelve populations of the Portuguese endangered medicinal plant Mentha cervina L. Morphological variation was correlated with ecological conditions at the site of origin. Pulegone was the major essential oils compound in all of the populations collected at full flowering (68–83%), in different growing conditions (51–82%), and for all the developmental stages studied (47–82%). Although clusters were defined, the analysis revealed a high chemical correlation among all populations (Scorr ≥ 0.95%). Inter-simple sequence repeats markers were used to assess the population structure and genetic variation. Populations exhibited a relatively low genetic diversity (PPB = 14.3–64.6%, He = 0.051–0.222, I = 0.076–0.332), with high structuring between them (GST = 0.51). However, the genetic diversity at species level was relatively high (PPB = 97.7%; He = 0.320). The levels and patterns of genetic diversity were assumed to result largely from a combination of evolutionary history and its unique biological traits, such as breeding system, clonal growth, low capacity of dispersion and habitat fragmentation. The relatively low genetic diversity in the populations analyzed indicates that the maintenance of their evolutionary potential is at risk if population sizes are maintained and if there is no protection of the habitats. |
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| Keywords: | Morphological trait Essential oil Genetic diversity ISSRs Conservation genetics |
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