Genetic diversity in Vicia amoena (Fabaceae) germplasm resource in China using SRAP and ISSR markers |
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| Institution: | 1. Dipartimento di Biologia, Università di Napoli Federico II, Naples, Italy;2. Stazione Zoologica Anton Dohrn, Naples, Italy;3. CNR-IAS, Genoa, Italy;1. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;2. The Specimen Museum of Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;3. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The genetic diversity in 17 germplasms of Vicia amoena L. from north China was analyzed using SRAP and ISSR markers. Three hundred and sixty-eight (94.11%) polymorphic bands (211 and 157 obtained from 20 pairs of SRAP and ISSR primers, respectively) were scored. Although SRAP was more effective than ISSR markers with higher PIC, RP and larger variation range of genetic distance, both the markers were useful for assessing V. amoena genetic diversity. Cluster analysis showed that the 17 germplasms were clustered into 5 groups. The results of principal coordinate analysis supported UPGMA clustering. The germplasms from source areas where the annual average temperature ranged from ?1.0 to 5 °C exhibited the highest level of genetic diversity with the highest PPI, I and H. These results have important implications in genome mapping, breeding purposes, and germplasm conservation. |
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| Keywords: | Genetic diversity SRAP markers ISSR markers |
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