Development of twenty-one polymorphic tetranucleotide microsatellite loci for Schizothorax o'connori and their conservation application |
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| Affiliation: | 1. Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China;2. Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, PR China;3. School of Biological Sciences, Victoria University of Wellington, P O Box 600, Wellington 6140, New Zealand;1. Korea Association of Health Promotion, Seoul 07653, Republic of Korea;2. Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of Korea;3. Department of Parasitology, Korea University College of Medicine, Seoul 02841, Republic of Korea;4. Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Republic of Korea;5. Department of Hygiene and Prevention, Ministry of Public Health, Vientiane, Lao Democratic People’s Republic;1. Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China;2. State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610065, China;3. Guizhou Survey/Design Research Institute for Water Resources and Hydropower, Guiyang 550002, China;4. Guizhou Engineering Technology Research Center for Exploitation and Utilization of Water Resources in Karst Region, Guiyang 550002, China;5. GNSS Research Center, Wuhan University, Wuhan 430079, China |
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| Abstract: | Schizothorax o'connori, of the subfamily Schizothoracinae, is an endemic fish in the Yarlung Tsangpo River in Tibet, China. To evaluate the population genetic diversity as a means of devising conservation strategies, a total of 21 polymorphic tetranucleotide microsatellite loci were isolated from S. o'connori using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. The characterization of these loci was assessed against a panel of 42 individuals. The number of alleles per locus ranged from 4 to 29 with an average of 17.76. Expected locus-specific heterozygosity and the multilocus Shannon–Wiener diversity index ranged from 0.114 to 0.951 and from 0.280 to 3.131, with an average of 0.851 and 2.338, respectively. The cross-species amplification and applicability of these loci were tested against the two other Schizothorax species from the same river. These microsatellite loci will be valuable for further studies of population genetic diversity and genetic structure, and assessments of the artificial propagation release effect of S. o'connori and other related species. |
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| Keywords: | Microsatellites Tetranucleotides Genetic diversity Tetraploid fish Schizothoracinae Conservation |
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