Molecular phylogeny of Trissolcus species (Hymenoptera: Scelionidae) |
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| Institution: | 1. Ankara University Faculty of Agriculture, Ankara, Turkey;2. Suleyman Demirel University Faculty of Agriculture, Isparta, Turkey;1. Laboratório de Biologia Comparada e Abelhas (LBCA), Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes, 3900, 14040-901, Ribeirão Preto, São Paulo, Brazil;2. Universidade Federal do Parana, Departamento de Zoologia, Laboratorio de Biologia Comparada de Hymenoptera, Caixa Postal 19020, 81531-980, Curitiba, Paraná, Brazil;1. Graduate Interdisciplinary Program in Entomology and Insect Science, 410 Forbes, University of Arizona, Tucson, AZ 85721, USA;2. Department of Entomology, ARO, Newe Ya''ar Research Center, P.O. Box 1021, Ramat Yishay, Israel;3. Department of Entomology, 410 Forbes, University of Arizona, Tucson, AZ 85721, USA;1. National Institute of Materials Physics, 405A Atomistilor Street, PO Box MG-7, Magurele, Bucharest 077125, Romania;2. P. Poni Institute of Macromolecular Chemistry,41 A Gr. Ghica Voda Alley, 700487 Iasi, Romania;3. National Institute for Laser, Plasma and Radiation Physics, Str. Atomistilor, Nr. 409, PO Box MG-36, Magurele, Bucharest 077125, Romania;4. University of Bucharest, Faculty of Physics, 405 Atomistilor Street, PO Box MG-11, Magurele, Bucharest 077125, Romania;5. LPHIA, UBL – Angers University, 2.Bd. Lavoisier, 49045, France;1. Department of Physics, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran;2. Department of Physics, Science and Research Branch, Islamic Azad University, Fars, Iran;1. School of Biological Sciences, The University of Queensland, Brisbane, Queensland, 4072, Australia;2. Department of Zoology, University of Otago, P.O. Box 56, Dunedin, 9054, New Zealand;3. Department of Agricultural Biotechnology, Faculty of Agriculture, ISUBU, Isparta, 32260, Turkey;4. Department of Agriculture and Fisheries, EcoSciences Precinct, GPO Box 267, Brisbane, Queensland, 4001, Australia |
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| Abstract: | Trissolcus species (Hymenoptera: Scelionidae) are the most promising biological control agents against sunn pest. The accurate identification of natural enemies is crucial in order to develop successful biological control programs. This paper presents phylogenetic analyses of Trissolcus species based on data sets consisting of 18S, 28S, ITS1, ITS2 and cytochrome oxidase subunit I (COI) genes. The most commonly used genetic loci in Trissolcus species identification is the cytochrome oxidase I gene (COI). Also restriction fragment length polymorphism analyses of PCR amplified COI gene have been developed to discriminate closely related species. We suggest that Trissolcus grandis Thompson and Trissolcus semistriatus Nees split significantly into two different genetic groups while there is another species diverging from T. semistriatus which is decsribed as ‘Trissolcus flavotibialis Kocak & Guz, n. sp.’ |
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