Induction and Characterization of Adventitious Roots Directlyfrom the Explants of <Emphasis Type="Italic">Panax notoginseng</Emphasis>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Induction and Characterization of Adventitious Roots Directlyfrom the Explants of Panax notoginseng

Authors:	Xianfu?Gao,Chunbao?Zhu,Wei?Jia author-information" > author-information__contact u-icon-before" > mailto:weijia@sjtu.edu.cn" title=" weijia@sjtu.edu.cn" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Wenyuan?Gao,Mingfeng?Qiu,Yongyu?Zhang,Peigen?Xiao

Affiliation:	(1) School of Pharmacy, Shanghai Jiao Tong University, 200030 Shanghai, China;(2) Shanghai Institute of Pharmaceutical Industry, 200040 Shanghai, China;(3) College of Pharmaceutical Science and Technology, Tianjin University, 300072 Tianji, China;(4) Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, 100094 Beijing, China

Abstract:	Adventitious roots from leafstalks and lateral roots were obtained directly from explants of Panax notoginseng. The lateral root explants were more sensitive to the induction of adventitious roots using indole-3-butyric acid. HPLC analysis of saponins extracted from the adventitious roots indicated that several protopanaxatriol saponins were present but ginsenoside Rd was missing, compared with the saponins extracted from the raw herbs. The dry weight of primary adventitious root culture of Panax notoginseng increased 5.25 times during multiplication in a classical shaking-flask system, suggesting that it is a culture system with great potential for scale-up. Revisions requested 13 July 2005; Revisions received 1 September 2005

Keywords:	adventitious roots ginseng indole-3-butyric acid morphogenesis Panax notoginseng saponin
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