Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum |
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Authors: | Peter Klauth Srinivas Reddy Pallerla Dolores Vidaurre Carla Ralfs Volker F Wendisch Siegfried M Schoberth |
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Institution: | (1) Institute of Agrosphere (ICG-IV), Research Center Juelich, Juelich, Germany;(2) Institute of Biotechnology (IBT-1), Research Center Juelich, Juelich, Germany;(3) Institute of Molecular Microbiology and Biotechnology (IMMB), Westfalian Wilhelms University, Muenster, Germany;(4) Present address: Institute of General Zoology and Genetics, Westfalian Wilhelms University, Muenster, Germany;(5) Present address: Casilla 2, Santa María, San Felipe, Chile |
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Abstract: | Corynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth conditions. Under phosphate-limiting conditions, C. glutamicum did not accumulate poly P, but it rebuilt its poly P storages when phosphate became available. The poly P content of C. glutamicum growing on glucose minimal medium with sufficient phosphate varied considerably during growth. While the poly P content was minimal in the midexponential growth phase, two maxima were observed in the early exponential growth phase and at entry into the stationary growth phase. Cells in the early exponential growth phase primarily contained granular poly P, while cells entering the stationary growth phase contained soluble, cytosolic poly P. These results and those obtained for C. glutamicum cells cultivated under hypo- or hyperosmotic conditions or during glutamate production revealed that the poly P content of C. glutamicum and the partitioning between cytosolic and granular forms of poly P are dynamics and depend on the growth conditions. |
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Keywords: | Poly P Volutin granules DAPI 31P NMR Phosphate starvation Osmoshock Ethambutol Tween 40 Glutamate production Digital image analysis Corynebacterium glutamicum |
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