Presence of allotypic determinants on polypeptide subunits of rabbit gamma globulin

Inhibition of passive haemagglutination showed the presence of the allotypic specificities Aa1 Aa2, Aa3, Ab4, Ab5 and Ab6 on polypeptide subunits of rabbit IgG belonging to the phenotypes Aa1-3/Ab4-4 Aa2-2/Ab4-4, Aa3-3/Ab4-4, Aa3-3/Ab4-5 and Aa3-3/Ab4-6, prepared by oxidative sulphitolysis followed by isolation on Sephadex G-100 in 6m urea and 0.05m formic acid. The determinants Aa1, Aa2 and Aa3 were found only on H chains and Ab4, Ab5 and Ab6 only on L chains. Of the latter, Ab4 and Ab5 were found in both fractions, i.e. L₁ and L₂, of the light chains, while Ab6 was found only in fraction L₁.